MylcMAT : Evaluation Kit for Monocyte Activation Test (MAT)

HOMEProduct/ServiceMylcMAT : Evaluation Kit for Monocyte Activation Test (MAT)

Developed using regenerative medicine technologies,
aMylc offers a transformative approach to MAT testing.

aMylc for MAT
aMylc cells (Myeloid lineage cells) are immortalized myeloid cells developed by isolating monocytes from human donor peripheral blood.
aMylc has 3 main features:
1. Higher cytokine production response to stimulation compared to other cells. (Limit of Detection (LOD) < 0.025 EU/mL)
2. Production in large batches enables stable evaluation and reproducibility
3. Unique immortalized cell manufacturing technology ensures consistent production and supply.
These 3 features makes aMylc cells suitable for MAT, which requires uniform and highly accurate results.

How aMylc for MAT is produced
How to use MylcMAT
Why is [MylcMAT] necessary?
MylcMAT Kit
Introduction to the Next-Generation MylcMAT (MylcMAT Rapid Kit)

How aMylc for MAT is produced

aMylc refers to Mylc cells derived from peripheral blood mononuclear cells (PBMCs) and induced as described above.
For MAT use, aMylc is produced from PBMCs that show high reactivity to microbial components.

How to use MylcMAT

Why is [MylcMAT] necessary?

Many MAT products have been developed using PBMCs or monocyte-derived cell lines. However, these models can often struggle with low responsiveness to microbial components, limited reproducibility, and limited supply.

At MiCAN, we address these issues with our proprietary immortalized monocyte cell line (aMylc), developed by isolating monocytes from healthy donor blood and applying our regenerative medicine technology (primary cell processing, cells expansion and immortalization techniques).

aMylc replicates the reactivity of fresh blood-derived cells, while enabling large-scale, consistent production as an immortalized cell line. This allows for highly reproducible MAT testing over extended periods.

MylcMAT Kit

Assessment principle

By using aMylc specifically screened for MAT, we achieved highly sensitive and consistent detection of non-endotoxin pyrogens (NEPs), surpassing the performance of conventional MAT cell preparations.

As an animal-free testing method, aMylc-based assays are free of animal-derived components(Like Fetal Bovine Serum, FBS), not only during testing, but also through the cells production and manufacturing process.

Introduction to the Next-Generation MylcMAT (MylcMAT Rapid Kit)

With the European Pharmacopoeia's decision to phase out the Rabbit Pyrogen Test (RPT) by 2025, the Monocyte Activation Test (MAT) is gaining recognition as a recommended alternative for pyrogen testing. However, most current MAT workflows require a two-day evaluation period, limiting their use for routine testing.
To overcome this, MiCAN is developing the MylcMAT Rapid Kit, designed to complete testing within a single day.
This advancement aims to facilitate the widespread implementation of MAT, aligning with the European regulatory shift towards in vitro testing methods.

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